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產(chǎn)品英文名稱(chēng)：StemRD W3A-H-025 WNT-3a,human,25ug
產(chǎn)品中文名稱(chēng)：StemRD W3A-H-025人源WNT-3a 蛋白因子，25ug
產(chǎn)品信息
Wnt糖蛋白是存在于多種生物體內(nèi)的一種細(xì)胞外配體，Wnt作為形態(tài)發(fā)生素通過(guò)激發(fā)細(xì)胞內(nèi)遠(yuǎn)離信號(hào)發(fā)送區(qū)域的濃度依賴(lài)反應(yīng)控制胚胎形態(tài)發(fā)育。Wnt通路調(diào)節(jié)動(dòng)物發(fā)育過(guò)程中的多個(gè)重要環(huán)節(jié)，例如細(xì)胞增殖、細(xì)胞遷徙及細(xì)胞分化。Wnt蛋白通過(guò)自分泌或旁分泌作用與位于細(xì)胞膜上的受體相結(jié)合，激活細(xì)胞內(nèi)信號(hào)通路調(diào)節(jié)靶基因的表達(dá)，對(duì)細(xì)胞的增殖、分化、遷移、極性化和凋亡起到重要作用。
StemRD W3A-H-025B WNT-3a,human,25ug 產(chǎn)品簡(jiǎn)介
StemRD已研發(fā)出一種新穎的表達(dá)和純化諸如StemRD W3A-H-025B WNT-3a,human,25ug 蛋白等“困難"蛋白的*方法。我們*的處理方法能得到純度和活性均于市場(chǎng)方法生產(chǎn)的WNT蛋白。StemRD W3A-H-025B WNT-3a,human,25ug 蛋白絕不含有現(xiàn)今市場(chǎng)普遍使用的WNT蛋白中含有的某些非特異性成分。
WNT-3a 干細(xì)胞生長(zhǎng)分化因子是WNT 信號(hào)蛋白家庭的成員之一，在胚胎成長(zhǎng)和成體組織維護(hù)方面起關(guān)鍵作用。WNT-3a 干細(xì)胞生長(zhǎng)分化因子是典型的通過(guò)β-聯(lián)蛋白通路發(fā)送信號(hào)。已有規(guī)格的WNT-3a是一個(gè)包含328個(gè)氨基酸殘基的單鏈蛋白質(zhì)。由于糖基化，WNT-3a 在非還原條件下用SDS-PAGE處理會(huì)明顯損失掉38-41 kDa分子量。StemRD公司生產(chǎn)的WNT-3a干細(xì)胞生長(zhǎng)分化因子是用人體細(xì)胞系中Wnt-3a cDNA 在無(wú)蛋白培養(yǎng)基上過(guò)度表達(dá)得到。使用StemRD W3A-H-025B WNT-3a,human,25ug *的純化方法，區(qū)別于其它已發(fā)表的純化方法，提高蛋白純度。
StemRD W3A-H-025B人源WNT-3a 蛋白因子，25ug 產(chǎn)品特性
?StemRD W3A-H-025B人源WNT-3a 蛋白因子，25ug 干細(xì)胞生長(zhǎng)分化因子使用LC/MS 層析，純度大于98%，結(jié)構(gòu)經(jīng)過(guò)NMR驗(yàn)證
?StemRD W3A-H-025B人源WNT-3a 蛋白因子，25ug 干細(xì)胞生長(zhǎng)分化因子生物活性認(rèn)證：經(jīng)過(guò)生物試驗(yàn)驗(yàn)證，優(yōu)于其它同類(lèi)型產(chǎn)品
? WNT-3a 干細(xì)胞生長(zhǎng)分化因子是典型的通過(guò)β-聯(lián)蛋白通路發(fā)送信號(hào)
StemRD W3A-H-025B人源WNT-3a 蛋白因子，25ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.
StemRD W3A-H-025B WNT-3a,human,25ug 產(chǎn)品英文描述
We express and purify StemRD W3A-H-025B WNT-3a,human,25ug proteins by using a proprietary process that is distinctive from the published protocol. Our method results inStemRD W3A-H-025B WNT-3a,human,25ug proteins that are purer and more active than market-leading products which have been associated with non-specific artifacts.
WNT-3a is a member of the WNT family of signaling proteins that play key roles in embryonic development and the maintenance of adult tissues. WNT-3a is a prototypic canonical WNT that signals through the β-catenin pathway. The predicted size of human WNT-3a is a monomeric protein containing 328 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of 38 - 41 kDa on SDS-PAGE under non-reducing conditions. StemRD’s W3A-H-025B WNT-3a,human,25ug,is produced from a human cell line overexpressing human Wnt-3a cDNA in protein-free medium.Purification is done by a proprietary process that is distinct from the published method.
WNT all compounds are:
1. >98% pure by LC/MS analysis, and structurally verified by NMR.
2. Bioactivity Certified: tested for biological activities, an advent-age over other supplies.
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