Anti-ATP5O [4C11C10D12] antibody (ab110276)抗ATP5O [4C11C10D12]抗體產(chǎn)品信息：

• Product nameAnti-ATP5O [4C11C10D12] antibodySee all ATP5O primary antibodies ...
• Description

  Mouse monoclonal [4C11C10D12] to ATP5O

• Tested applicationsICC/IF, WB, Flow Cyt, IHC-P more details
• Species reactivity

  Reacts with: Cow, Human, African Green Monkey

• Immunogen

  Recombinant mature Human ATP5O.

• Positive control
  • Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: HBS

• Concentration 100 µg at 1 mg/ml

• PurityIgG fraction
• Purification notesNear homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
• Clonality Monoclonal
• Clone number4C11C10D12
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria
  • Signal Transduction
  • Metabolism
  • Mitochondrial
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex V
  • Metabolism
  • Types of disease
  • Cancer

Applications

Our Abpromise guarantee covers the use of ab110276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
• Sequence similaritiesBelongs to the ATPase delta chain family.
• Cellular localizationMitochondrion. Mitochondrion inner membrane.
• Information by UniProt

• Database links

  • Entrez Gene: ATP5O Cow
  • Entrez Gene: 539 Human
  • Omim: 600828 Human
  • SwissProt: P13621 Cow
  • SwissProt: P48047 Human
  • Unigene: 49172 Cow
  • Unigene: 409140 Human

• Alternative names

  • ATP synthase O subunit mitochondrial precursor antibody
  • ATP synthase subunit O antibody
  • ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody

Anti-ATP5O [4C11C10D12] antibody images

• 

  Western blot - ATP5O antibody [4C11C10D12] (ab110276)

  All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 0.5 µg/ml
  Lane 1 : Human heart mitochondria at 5 µg
  Lane 2 : Bovine heart mitochondria at 1 µg
  Predicted band size : 23 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)

  Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.

• 

  Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)

  Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

• 

  Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)

  HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

• 

  Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)

  All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 1 µg/ml
  Lane 1 : HDFn (human) cell lysates at 20 µg
  Lane 2 : COS7 (monkey) cell lysates at 20 µg
  Lane 3 : H4IIE (rat) cell lysates at 20 µg
  Lane 4 : MEF (mouse) cell lysates at 20 µg
  Lane 5 : bovine heart mitochondria lysates at 5 µg
  Predicted band size : 23 kDa

  Secondary antibody: Goat-Anti-Mouse-IR800 1:4000

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