InvivoGen pnifty2-luc pNiFty2-Luc
pNiFty2-Luc

pNiFty2-Luc plasmid is composed of three key elements: an ELAM proximal promoter, five NF-κB repeated transcription factor binding sites (TFBS) and a Luc (Luciferase) reporter gene.

pNiFty2-Luc  plasmid is selectable with Zeocin™ in both E. coli and mammalian cells, and can be used to generate stable clones.

Contents update notification

All InvivoGen plasmids are now provided as lyophilized DNA.
Description：
Minimal promoter

The proximal promoters are shorter than 500 bp and contain transcription factor binding sites. Upon stimulation in 293 cells, their expression level remains undetectable. With the addition of repeated TFBS, the proximal promoters become inducible by the appropriate stimulus and drive the expression of the reporter gene.

ELAM promoter: the proximal promoter of the endothelial cell-leukocyte adhesion molecule (ELAM-1; E-selectin) gene contains three NF-κB sites and is truly NF-kB specific, as it lacks an AP1/CREB site found in the full-length promoter [1, 2].

Transcription factor binding sites (TFBS)

NF-kB binding site: Nuclear factor (NF)-κB is a “rapid-acting” primary transcription factor activated by a wide variety of PRRs. NF-κB is a protein complex that belongs to the Rel-homology domain-containing protein family. The prototypical NF-κB is composed of the p65(RelA) and p50 subunits [3]. NF-κB binds specific decameric DNA sequences (GGGRNNYYCC, R-purine Y=pyrimidine) and activates genes involved in the regulation of the innate and adaptative immune response.

Reporter Genes

Luc reporter gene: The firefly luciferase gene is a highly sensitive reporter gene and thus is ideal for detecting low-level gene expression. Luc activity can be quantified in cell extracts by using kits commercially available from other companies.

1. Schindler U., Baichwal VR., 1994. Three NF-kappa B binding sites in the human E-selectin gene required for maximal tumor necrosis factor alpha-induced expression. Mol Cell Biol. 14(9):5820-31.
2. Jensen LE. & Whitehead AS., 2003. ELAM-1/E-selectin promoter contains an inducible AP-1/CREB site and is not NFkB-specific. Biotechniques 35:54-58.
3. Kawai T. & Akira S., 2007. Signaling to NF-kappaB by Toll-like receptors. Trends Mol Med. 13(11):460-9.
Details：

InvivoGen pnf3-lc3 pNiFty3-A-Lucia 20 µg
InvivoGen pnf3-lc6 pNiFty3-AN-Lucia 20 µg
InvivoGen pnf3-sp6 pNiFty3-AN-SEAP 20 µg
InvivoGen pnf3-sp3 pNiFty3-A-SEAP 20 µg
InvivoGen pnf3-lc7 pNiFty3-IAN-Lucia 20 µg
InvivoGen pnf3-sp7 pNiFty3-IAN-SEAP 20 µg
InvivoGen pnf3-lc4 pNiFty3-I-Lucia 20 µg
InvivoGen pnf3-sp4 pNiFty3-I-SEAP 20 µg
InvivoGen pnf3-lc1 pNiFty3-Lucia 20 µg
InvivoGen pnf3-lc2 pNiFty3-N-Lucia 20 µg
InvivoGen pnf3-sp2 pNiFty3-N-SEAP 20 µg
InvivoGen pnf3-sp1 pNiFty3-SEAP 20 µg
InvivoGen pnf3-lc8 pNiFty3-TAN-Lucia 20 µg
InvivoGen pnf3-sp8 pNiFty3-TAN-SEAP 20 µg
InvivoGen pnf3-lc5 pNiFty3-T-Lucia 20 µg
InvivoGen pnf3-sp5 pNiFty3-T-SEAP 20 µg
InvivoGen pnifty-luc pNiFty-Luc 20 µg
InvivoGen pnifty-seap pNiFty-SEAP 20 µg
InvivoGen tlrl-pau Poly(A:U) 10 mg
InvivoGen tlrl-pan Poly(dA) 200 µg
InvivoGen tlrl-pac Poly(dA)/LyoVec™ 100 µg
InvivoGen tlrl-patc Poly(dA:dT) / LyoVec™ 100 µg
InvivoGen tlrl-patn Poly(dA:dT) naked 200 µg
InvivoGen tlrl-patn-1 Poly(dA:dT) naked 1 mg
InvivoGen tlrl-patr Poly(dA:dT) Rhodamine 10 µg
InvivoGen tlrl-pgcc Poly(dG:dC) / LyoVec™ 100 µg
InvivoGen tlrl-pgcn Poly(dG:dC) naked 200 µg
InvivoGen tlrl-pt17 Poly(dT) 100 nmol
InvivoGen tlrl-pic Poly(I:C) (HMW) 10 mg
InvivoGen tlrl-pic-5 Poly(I:C) (HMW) 50 mg
InvivoGen tlrl-piclv Poly(I:C) (HMW) / LyoVec™ 100 µg
InvivoGen tlrl-picb Poly(I:C) (HMW) Biotin 10 µg
InvivoGen tlrl-picf Poly(I:C) (HMW) Fluorescein 10 µg
InvivoGen tlrl-picr Poly(I:C) (HMW) Rhodamine 10 µg
InvivoGen vac-pic Poly(I:C) (HMW) VacciGrade 10 mg
InvivoGen tlrl-picw Poly(I:C) (LMW) 25 mg
InvivoGen tlrl-picw-250 Poly(I:C) (LMW) 250 mg
InvivoGen tlrl-picwlv Poly(I:C) (LMW) / LyoVec™ 100 µg
InvivoGen tlrl-piwr Poly(I:C) (LMW) Rhodamine 10 µg
InvivoGen tlrl-pmb Polymyxin B 100 mg
InvivoGen ant-pm-1 Primocin™ 500 mg (10 x 1 ml)
InvivoGen ant-pm-2 Primocin™ 1 g (1 x 20 ml)
InvivoGen prom-test PromTest 10 x 5 µg
InvivoGen gel-agg-2 Protein G / Agarose 2 ml
InvivoGen gel-agg-5 Protein G / Agarose 5 ml
InvivoGen gel-protl-10 Protein L / Agarose 10 ml
InvivoGen gel-protl-2 Protein L / Agarose 2 ml
InvivoGen psetb-lacz pSELECT-blasti-LacZ 20 µg
InvivoGen psetb-mcs pSELECT-blasti-mcs 20 µg
InvivoGen psetb-cgfp pSELECT-CGFP-blasti 20 µg
InvivoGen psetz-cgfp pSELECT-CGFP-zeo 20 µg
InvivoGen psetb-cha pSELECT-CHA-blasti 20 µg
InvivoGen psetz-cha pSELECT-CHA-zeo 20 µg
InvivoGen psetb-chis pSELECT-CHis-blasti 20 µg
InvivoGen psetz-chis pSELECT-CHis-zeo 20 µg
InvivoGen psetz-gfplc3 pSELECT-GFP-hLC3 20 µg
InvivoGen psetz-gfpmlc3 pSELECT-GFP-mLC3 20 µg
InvivoGen psetgz-lacz pSELECT-GFPzeo-LacZ 20 µg
InvivoGen psetgz-mcs pSELECT-GFPzeo-mcs 20 µg
InvivoGen pseth-lacz pSELECT-hygro-LacZ 20 µg
InvivoGen pseth-mcs pSELECT-hygro-mcs 20 µg
InvivoGen psetn-lacz pSELECT-neo-LacZ 20 µg
InvivoGen psetn-mcs pSELECT-neo-mcs 20 µg
InvivoGen psetb-ngfp pSELECT-NGFP-blasti 20 µg
InvivoGen psetz-ngfp pSELECT-NGFP-zeo 20 µg
InvivoGen psetb-nha pSELECT-NHA-blasti 20 µg
InvivoGen psetz-nha pSELECT-NHA-zeo 20 µg
InvivoGen psetb-nhis pSELECT-NHis-blasti 20 µg
InvivoGen psetz-nhis pSELECT-NHis-zeo 20 µg
InvivoGen psetb-nlucia pSELECT-NLucia-blasti 20 µg
InvivoGen psetz-nlucia pSELECT-NLucia-zeo 20 µg
InvivoGen psetp-lacz pSELECT-puro-LacZ 20 µg
InvivoGen psetp-mcs pSELECT-puro-mcs 20 µg
InvivoGen psetz-fcyfur pSELECT-zeo-FcyFur 20 µg
InvivoGen psetz-zgfpbsr pSELECT-zeo-GFPBsr 20 µg
InvivoGen psetz-zgfpsh pSELECT-zeo-GFPSh 20 µg
InvivoGen psetz-hseap pSELECT-zeo-hSEAP-CpG-free 20 µg
InvivoGen psetz-hsv1tk pSELECT-zeo-HSV1tk 20 µg
InvivoGen psetz-hsv1tksh pSELECT-zeo-HSV1tkSh 20 µg
InvivoGen psetz-lacz pSELECT-zeo-LacZ 20 µg
InvivoGen psetz-lacznls pSELECT-zeo-LacZnls 20 µg
InvivoGen psetz-laczsh pSELECT-zeo-LacZSh 20 µg
InvivoGen psetz-lucia pSELECT-zeo-Lucia 20 µg
InvivoGen psetz-lucsh pSELECT-zeo-LucSh 20 µg
InvivoGen psetz-mcs pSELECT-zeo-mcs 20 µg
InvivoGen psetz-mseap pSELECT-zeo-mSEAP-CpG-free 20 µg
InvivoGen psetz-seap pSELECT-zeo-SEAP 20 µg
InvivoGen psirna42-hdhx9 psiRNA-DHX9 20 µg
InvivoGen psirna3z21-dsred2 psiRNA-DsRed2 20 µg
InvivoGen ksirna3z21-dsred2 psiRNA-DsRed2 Kit 1 kit
InvivoGen ksirna4-gz3 psiRNA-DUO 1 kit
InvivoGen psirna3z21-egfp psiRNA-EGFP 20 µg
InvivoGen ksirna3z21-egfp psiRNA-EGFP Kit 1 kit
InvivoGen ksirna3-b21 psiRNA-h7SK G1 Blasti 1 kit
InvivoGen ksirna4-gz21 psiRNA-h7SK G1 GFPzeo 1 kit
InvivoGen ksirna3-h21 psiRNA-h7SK G1 Hygro 1 kit
InvivoGen ksirna3-n21 psiRNA-h7SK G1 Neo 1 kit
InvivoGen ksirna3-z21 psiRNA-h7SK G1 Zeo 1 kit
InvivoGen psirna42-ha20 psiRNA-hA20 20 µg
InvivoGen ksirna42-ha20 psiRNA-hA20 Kit 1 kit
InvivoGen psirna42-hager psiRNA-hAGER 20 µg
InvivoGen ksirna42-hager psiRNA-hAGER Kit 1 kit
InvivoGen psirna42-haim2 psiRNA-hAIM2 20 µg
InvivoGen ksirna42-haim2 psiRNA-hAIM2 Kit 1 kit
InvivoGen psirna42-hasc psiRNA-hASC 20 µg
InvivoGen ksirna42-hasc psiRNA-hASC Kit 1 kit
InvivoGen psirna42-hatf3 psiRNA-hATF3 20 µg
InvivoGen ksirna42-hatf3 psiRNA-hATF3 Kit 1 kit
InvivoGen psirna42-hatg5 psiRNA-hATG5 20 µg
InvivoGen ksirna42-hatg5 psiRNA-hATG5 Kit 1 kit
InvivoGen psirna42-hbaf47 psiRNA-hBAF47 20 µg
InvivoGen ksirna42-hbaf47 psiRNA-hBAF47 Kit 1 kit
InvivoGen psirna42-hbcl10 psiRNA-hBCL10 20 µg
InvivoGen ksirna42-hbcl10 psiRNA-hBCL10 Kit 1 kit
InvivoGen psirna42-hbcl2 psiRNA-hBcl2 20 µg

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